Urine Drug Test Diagram and Panels Explained

Each One Step Drug Test card consists of 1 or more Panels. Each panel represents 1 drug category detected. A 1-Panel Drug Test Card detects 1 drug category, for example: amphetamine (AMP panel). A Multi-Panel Drug Test Card detects multiple drug categories, up to 12.

Each drug test panel contains a Test Result Viewing Window and a corresponding Drug Test Strip for absorbing the urine sample and transporting it to the Viewing Window for a reading. A separate test result is displayed for each panel/drug category. (See diagram below.)

Drug Test Diagram

The 6-panel One Step Drug Test, shown above, detects 6 drug categories. The absorbent Drug Test Strips are dipped in urine and removed. Within 5 minutes, results are displayed in each Viewing Window. 2 Red Lines = Negative Test Result. 1 Red Line = Positive Test Result.

A detailed Product Insert Sheet is included with each order, containing complete Instructions and Specifications.

Intended Use

The One Step Drug Screen Test Card is a lateral flow, chromatographic immunoassay for the qualitative detection of drugs and/or drug metabolites in human urine at specified cutoff concentrations. For healthcare professionals, including professionals at point of care sites. For in vitro diagnostic use only.

This assay provides only a preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS) are the preferred confirmatory methods. Clinical consideration and professional judgment should be applied to any drug of abuse test result, particularly when preliminary positive results are indicated.

Drug Test Reagents

Each test line contains anti-drug mouse monoclonal antibody and corresponding drug-protein conjugates. The control line contains goat anti-rabbit lgG polyclonal antibodies and rabbit lgG.

Drug Test Principle

The One Step Drug Screen Test Card is an immunoassay based on the principle of competitive binding. Drugs that may be present in the urine specimen compete against their respective drug conjugate for binding sites on their specific antibody.

During testing, a urine specimen migrates upward by capillary action. A drug, if present in the urine specimen below its cutoff concentration, will not saturate the binding sites of its specific antibody. The antibody will then react with the drug-protein conjugate and a visible colored line will appear in the test line region (T) of the specific drug strip. (See Drug Test Diagram above for reference.) The presence of drug above the cutoff concentration will saturate all the binding sites of the antibody. Therefore, the colored line will not form in the test line region (T).

A drug-positive urine specimen will not generate a colored line in the specific test line region (T) of the strip because of drug competition, while a drug-negative urine specimen or a specimen containing a drug concentration lower than the cutoff will generate a line in the test line region (T) because of the absence of drug competition.

To serve as a procedural control, a colored line will always appear at the control line region (C), indicating that proper volume of specimen has been added and membrane wicking has occurred.